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Cite YouScope

Lang, M., Rudolf, F., & Stelling, J. (2012).
Use of YouScope to Implement Systematic Microscopy Protocols.
Current Protocols in Molecular Biology, 14-21.

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Mode of Operation

The following small interactive app exemplifies –in a simplified way– the interaction between the experimentalist and YouScope to set up a microplate measurement.

Measured Wells:

(Click to activate/deactivate)

Channels:

  • CFP, Exposure:
  • GFP, Exposure:

YouScope - The Microscope Control Software

Figure 1: YouScope supports multiple cameras.

YouScope is a free, open-source microscope control software compatible with most present-day automized microscopes. YouScope allows to easily automatize complex microscopy protocols using an user-friendly graphical interface. Besides a wide range of built-in configurable standard protocols, it is easily extendible to support specific custom protocols.

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News

17.08.2016 – Sixth beta of YouScope 2.0 available

YouScope R2016 Beta 6 is now available. Most of the changes in this version consider the support of non-rectangular microplates. Since a lot of the code base considering microplate measurements had to be changed, this version might not be as stable as the other beta releases. Choose Beta 5 or a later version if stability is most important for you.
New functionality/changes:

  • Support of custom, non-rectangular microplates. The microplates can have virtually any layout, including gaps, missing wells, different well sizes, and unregular positions.
  • Path optimizers were revisited: there are a few more options in the different optimizers, e.g. different metrics.
  • Complete refactoring of the microplate measurement: Position configuration should be significantly simpler, and configured positions can manually be changed afterwards.
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29.07.2016 – Fifth beta of YouScope 2.0 available

We just released the fifth beta version of YouScope 2.0. In this beta, we introduce new path optimizers for microplate measurements. These optimizers are 1.5- and 2-approximations of the traveling salesman problem, thus, typically return very good or even the optimal paths through the microplate. Important Note: The last beta release of YouScope (R2016 Beta 4) contained a serious bug which might lead to loss of data (overweiting of images during a measurement) when imaging different wells of a microplate havind different y-positions, but the same x-position. In this bug, the file-name generated by default for new images only includes the identifier of the y-position, but not of the x-position. We strongly recommend that anybody using the Beta 4 either switches to the new Beta 5 version, or downgrades to Beta 3. All versions can be found in our download area.

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15.06.2016 – Fourth beta of YouScope 2.0 available

We just released the fourth beta version of YouScope 2.0. In this beta, it is now possible to fully define how measurement data (images, meta-data) should be saved to the disk, by so called "custom save settings" (under Tools menu). Besides this, we fixed several smaller bugs.

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13.05.2016 – Third beta of YouScope 2.0 available

We just released the third beta version of YouScope 2.0. Besides several smaller bugfixes, we added support to measure cell sizes or similar in the LiveStream. Furthermore, support for SLIM microscopy as well as droplet based microfluidics was significantly extended. Also, a new red skin is available, supposingly being good for the eyes when starring at the screen for hours in a dark microscopy chamber. The third beta is stable, meaning that we recommend switching right now! You can download the latest release of Youscope in our download area.

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09.02.2016 – YouScope 2.0 coming

We are currently preparing the stable release of YouScope 2.0. As compared to the 1.X versions, YouScope 2.0 offers an exciting new user interface, with an revised LiveStream, functionality to view all images of a running or finished measurement, Skins (check it out!) and much more. We already released the second beta version of YouScope 2.0. As compared to the final version, we expect only minor changes/bug-fixes to still happen. The second beta is stable, meaning that we recommend switching right now! You can download the latest release of Youscope in our download area.

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